His-tag Polyclonal Antibody-BS67270

His-tag Polyclonal Antibody

Catalogue Numbers: BS67270-50, BS67270-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Host: Rabbit

Applications: WB, IP

All Applications: WB (1/1000 - 1/2000), IP (1/10 - 1/100)

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Extra Notes: Western blot analysis of Anti-His-tag Antibody against HEK293T cells transfected with vector overexpressing His tag (A) and untransfected (B).

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Immunogen: KLH-conjugated synthetic peptide HHHHHH.

Conjugate: Unconjugated

Modification: Unmodified