Product Description

His-tag Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA20771-50, RA20771-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Reactivity: Species independent

Host: Rabbit

Applications: WB, ELISA

Alternate Names: 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag

Clonality: Polyclonal

Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight: 15

Protein Molecular Weight (kDa): 15

Storage: -20°C/1 year

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Research Use Only