Product Description

His-tag Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA20771-50, RA20771-100

Citations, Manuals and MSDS Available upon request.

Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

Alternative Names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Applications: WB; ELISA

Species Cross-Reactivity: Species independent

Protein MW (KDa): 15

Source: Rabbit

Research Use Only