Product Description

Histone Deacetylase 3 (Phospho-S424) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64528-50, BS64528-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regμlation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone resμlts in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Histone Deacetylase 3 around the phosphorylation site of Serine 424.

Dilution: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Histone Deacetylase 3 (Phospho-S424) polyclonal antibody detects endogenous levels of Histone Deacetylase 3 protein only when phosphorylated at Ser424.

Molecular Weight: ~ 49 kDa

Pathway: Notch Signaling, Nuclear Receptor Signaling, Epigenetic Writers and Erasers of Histone H3,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O15379

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only