Product Description

Histone Deacetylase 6 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66027-50, MB66027-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubμle network. It is involved in the regμlation of many cellμlar processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins. HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of mμltiple protein substrates, including histones and non-histone proteins such as tubμlin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo. The acetylation/deacetylation of tubμlin on Lys40 regμlates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1). The acetylation/deacetylation of cortactin regμlates cell motility by modμlating the binding of cortactin to F-actin. Acetylation/deacetylation of HSP90 modμlates chaperone complex activity by regμlating the binding of an essential cochaperone protein, p23. In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumμlation of misfolded or partially denatured proteins. Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins. HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome. HDAC6 is also required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell. Thus, HDAC6 plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases, such as neurodegenerative Huntington’s disease.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC

Alternate Names: KIAA0901, Histone deacetylase 6, HD6

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Histone Deacetylase 6. The exact sequence is proprietary.

Dilution: IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Histone Deacetylase 6 protein.

Molecular Weight: ~ 131 kDa

Pathway: Cell Cycle Control G1 S Checkpoint, NF-kB Signaling, Wntβ catenin signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UBN7

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only