Histone H2A (Hydroxyl-Y39) Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9861M-50, BS9861M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04908/P28001/P35065/Q93077/Q99878
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:1000-1:2000
IHC: 1:50-1:200
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Global mass spectrometric analysis of histone H2A variants/subtypes and their modifications have only recently been carried out. Nine histone H2A subtypes have been identified, including H2A2A and H2A2B. The two main H2A variants, H2AO and H2AC, as well as H2AL, were either acetylated at Lys 5 or phosphorylated at Ser 1. For the replacement histone H2AZ, acetylation at Lys 4 and Lys 7 was found.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Histone H2A protein only when Hydroxyl at Tyr9.
Bioworld Molecular Weight: ~ 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Histone H2A (Hydroxyl-Y39) Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.
Alternative Name: Histone H2A type 1-B/E; HIST1H2AB; H2AFM; HIST1H2AE; H2AFA; Histone H2A type 1-C; HIST1H2AC; H2AFL; Histone H2A type 1-J; HIST1H2AJ; H2AFE
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Unmodified