Histone H2A.X Monoclonal Antibody-MB11029

Histone H2A.X Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11029-50, MB11029-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P16104

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of Histone H2A.X in HL-60 using Histone H2A.X antibody, and DAPI., Western blot analysis of Histone H2A.X in PC-12 lysates using Histone H2A.X antibody., Western blot analysis of Histone H2A.X in 3T3, Hela lysates using Histone H2A.X antibody, Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X

Immunogen: A synthetic peptide of human Histone H2A.X

Conjugate: Unconjugated

Modification: Unmodified