Histone H2A.X (phospho-S139) Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9844M-50, BS9844M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P16104
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:1000-1:5000
IHC: 1:50-1:200
Background: H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.
Bioworld Molecular Weight: ~ 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Histone H2A.X (phospho-S139) Rabbit mAb at 1:1000 dilution
Lane 1: HepG2 cell lysate–treated with etoposide
Lane 2: HepG2 cell lysate–untreated, Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Phospho-Histone H2A.X(S139) antibody. Counter stained with hematoxylin.
Alternative Name: Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Phosphorylation