Histone H2A.Z Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90640-50, BS90640-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04908(Human) EntrezGene:51788(Mouse) 58940(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ICC/IF
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Histone H2A.Z/H2A.F/Z (H2A/z) is a 128 amino acid protein encoded by the human gene H2AFZ. Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA sequentially in a left-handed super-helical turn to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, creating two nearly symmetrical halves by tertiary structure. H2A.Z/H2A.F/Z is a variant Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in tran-scription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of posttranslational modifications of histones, also called histone code, and nucleosome remodeling. H2A.Z/H2A.F/Z may be involved in the formation of constitutive heterochromatin and may be required for chromosome segregation during cell division.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Histone H2A.Z polyclonal antibody detects endogenous levels of Histone H2A.Z protein.
Bioworld Molecular Weight: 13 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Histone H2A.Z in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Alternative Name: HIST1H2AB, H2A histone family member Z H2A.z H2A/z H2afz H2AZ H2AZ_HUMAN Histone H2A.Z MGC117173
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified