Histone H2B (K24) Polyclonal Antibody-BS2568

Histone H2B (K24) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2568-50, BS2568-100

Sizes: 50µl, 100µl

Swiss-Prot: Q93079

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Histone H2B (K24) polyclonal antibody detects endogenous levels of Histone H2B type 1-H protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Histone H2B polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of Histone H2B (K24) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 18 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Histone H2B.

Conjugate: Unconjugated

Modification: Unmodified