Histone H2B Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90641-50, BS90641-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60814(Human) P10854(Mouse) Q64475(Mouse) Q00729(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Histone H2B polyclonal antibody detects endogenous levels of Histone H2B protein.
Bioworld Molecular Weight: 14 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: PC12, ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Hist1h2ba, H2B GL105, H2B histone family member O, H2B histone family member S, H2B.1, H2B.1 B, H2B.b, H2B.c, H2B.d, H2B.e, H2B.f, H2B.j, H2B.q, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/j, H2B/o, H2B/q, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFJ, H2BFO, H2BFQ, H2BFS, HIRIP2, HIST1H2BB, HIST1H2BD, HIST1H2BH, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST2H2BE, Histone H2B, Histone H2B type 1 B, Histone H2B type 1 D, Histone H2B type 1 H, Histone H2B type 1 L, Histone H2B type 1 M, Histone H2B type 1 N, Histone H2B type 2 E, histone protein,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified