Product Description

Histone H3.3 Polyclonal Antibody

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16641-50, BZ16641-100

Swiss-Prot: P84243

Host: Rabbit

Histone H3.3 Polyclonal Antibody-BZ16641

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Purification and Purity: Affinity Purified

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H3.3 in HeLaLysates; NIH/3T3Lysates using Histone H3.3 antibody., Immunohistochemistry analysis of paraffin-embedded mouse stomach using Histone H3.3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: H3F3; H3.3A

Immunogen: A synthesized peptide derived from human Histone H3.3

Modification: Unmodified

Conjugate: Unconjugated