Histone H3 (mono+di+methyl K79) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94075-50, BS94075-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P68431(Human) P84243(Human) Q16695(Human) Q6NXT2(Human) Q71DI3(Human) P68433(Mouse) Q6LED0(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Histone H3 (mono+di+methyl K79) polyclonal antibody detects endogenous levels of Histone H3 protein only when mono/di-methylated at K79.
Bioworld Molecular Weight: 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Histone H3(mono+di+tri methyl K79) on different lysates using anti-Histone H3(mono+di+tri methyl K79) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: CRC, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3(mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.
Alternative Name: Histone H3, H3 histone family, member J, FLJ92264, H3 histone family, member A, H3 histone family, member B, H3 histone family, member C, H3 histone family, member D, H3 histone family, member F, H3 histone family, member H, H3 histone family, member I, H3 histone family, member K, H3 histone family, member L, H3 histone, family 3A, H3.3A, H3/a, H3/b, H3/c, H3/d, h3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H31, H3F1K, H3F3, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, HIST3H3, Histone 1, H3a, Histone 1, H3b, Histone 1, H3c, Histone 1, H3d, Histone 1, H3e, Histone 1, H3f, Histone 1, H3g, Histone 1, H3h, Histone 1, H3i, Histone 1, H3j, Histone cluster 1, H3a, Histone cluster 1, H3b, Histone cluster 1, H3c, Histone cluster 1, H3d, Histone cluster 1, H3e, Histone cluster 1, H3f, Histone cluster 1, H3g, Histone cluster 1, H3i, Histone cluster 1, H3j, Histone H 3, Histone H3.1, Histone H3.2, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone H3/m, Histone H3/o,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Methylated