Histone H3 (mono methyl K18) Rabbit Monoclonal Antibody-BS9810M

Histone H3 (mono methyl K18) Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9810M-50, BS9810M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P68431/P84243/Q16695/Q6NXT2/Q71DI3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:5000 IHC/ICC/IF: 1:50-1:200

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Histone H3 protein only when Methyl at Lys18.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Histone H3 (mono methyl K18) Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate, Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H3(mono methyl K18) antibody. Counter stained with hematoxylin., ICC staining Histone H3(mono methyl K18) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Histone H3.1; Histone H3A; H3FA; Histone H3B; H3FL; Histone H3C; H3FC; Histone H3D; H3FB; Histone H3E; H3FD; Histone H3F; H3FI; Histone H3G; H3FH; Histone H3H; H3FK; Histone H3I; H3FF; Histone H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Methylated