Histone H3 (mono-methyl K36) Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9873M-50, BS9873M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P68431/P84243/Q16695/Q6NXT2/Q71DI3
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF
All Applications: WB: 1:1000-1:2000
ICC: 1:50-1:200
Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of histone H3 only when mono-methylated at Lys36.
Bioworld Molecular Weight: ~ 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Histone H3 (mono-methyl K36) Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate, ICC staining Histone H3(mono methyl K36) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Alternative Name: H3K36me1
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Methylated