Product Description

Histone H3 (MonoMethyl-K122) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67530-50, BS67530-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing mμltiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination . Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, 36, and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regμlation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair . Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins . In addition, acetylation of specific lysine residues creates docking sites for a protein modμle called the bromodomain, which binds to acetylated lysine residues . Many transcription and chromatin regμlatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation. Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression .

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Host: Rabbit

Applications: WB

Alternate Names: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST2H3A, HIST2H3C, H3F2, H3FM, HIST2H3D, Histone H3.2, Histone H3/m, Histone H3/o, H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3, H3F3C, Histone H3.3C, Histone H3.5

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H3 with a site at MonoMethyl-K122. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Histone H3 with a site at MonoMethyl-K122 protein.

Molecular Weight: ~ 17 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway，Histone Lysine Methylation Pathway，NF-kB Signaling，Signaling Pathways Activating p38 MAP Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P68431, Q71DI3, P84243, Q6NXT2

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only