Histone H3 Mouse Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9021-50, MB9021-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P68431
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:5000
IHC: 1:100-1:200
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Histone H3 and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Histone H3 Mouse mAb at 1:5000 dilution
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: 293 whole cell lysate
Lane 6: L929 whole cell lysate
Lane 7: F9 whole cell lysate
Lane 8: HepG2 whole cell lysate
Lane 9: MCF7 whole cell lysate
Lane 10: The kidney tissue lysate of Mouse
Lane 11: The brain tissue lysate of Mouse
Lane 12: The liver tissue lysate of Mouse
Lane 13: The liver tissue lysate of Rat, Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.
Alternative Name: Histone H3.1
Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of Histone H3.
Conjugate: Unconjugated
Modification: Unmodified