Histone H3 (TriMethyl-K9) Monoclonal Antibody-MB65950

Histone H3 (TriMethyl-K9) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB65950-50, MB65950-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P68431; Q71DI3; P84243

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB (1/1000 - 1/3000), IHC (1/200 - 1/500), IP (1/100 - 1/200)

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Histone H3 (TriMethyl K9) protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H3 (TriMethyl K9) expression in Hela (A) whole cell lysates., Immunohistochemical analysis of Histone H3 (TriMethyl K9) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunoprecipitation of Histone H3 (TriMethyl K9) from 0.5mg Hela whole cell extract lysate, using Anti-Histone H3 (TriMethyl K9) Antibody.

Alternative Name: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H3. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified