Histone H4 (acetyl K16) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94076-50, BS94076-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P62805(Human) P62806(Mouse) P62804(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Histone H4 (acetyl K16) polyclonal antibody detects endogenous levels of Histone H4 protein only when acetylated at K16.
Bioworld Molecular Weight: 11 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Histone H4 (acetyl K16) on SiHa cell using anti-Histone H4 (acetyl K16) antibody at 1/500 dilution., ICC staining Histone H4 (acetyl K16) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: , Histone gene cluster 1, H4A, Histone gene cluster 2, H4, dJ160A22.1, dJ160A22.2, dJ221C16.1, dJ221C16.9, FO108, H4, H4 histone family, member A, H4 histone family, member B, H4 histone family, member C, H4 histone family, member D, H4 histone family, member E, H4 histone family, member G, H4 histone family, member H, H4 histone family, member I, H4 histone family, member J, H4 histone family, member K, H4 histone family, member M, H4 histone family, member N, H4 histone, family 2, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4/p, H4, H4F2, H4F2iii, H4F2iv, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, HIST1 cluster, H4A, HIST1 cluster, H4B antibody HIST1 cluster, H4D antibody HIST2H4 antibody Hist4 cluster, H4 antibody Hist4h4 antibody histone 1, H4a antibody histone 1, H4c antibody histone 1, H4d antibody histone 1, H4f antibody histone 1, H4h antibody histone 1, H4i antibody histone 1, H4j antibody histone 1, H4k antibody histone 1, H4l antibody histone 2, H4a antibody histone 2, H4b antibody Histone 4 family, member M antibody histone 4, H4 antibody histone cluster 1, H4 antibody histone cluster 1, H4a antibody histone cluster 1, H4b antibody histone cluster 1, H4c antibody histone cluster 1, H4d antibody histone cluster 1, H4e antibody histone cluster 1, H4f antibody histone cluster 1, H4h antibody histone cluster 1, H4i antibody histone cluster 1, H4j antibody histone cluster 1, H4k antibody histone cluster 1, H4l antibody histone cluster 2, H4a antibody histone cluster 2, H4b antibody histone cluster 4, H4 antibody Histone family, member A antibody Histone family, member B antibody Histone family, member D antibody Histone family, member H antibody Histone family, member I antibody Histone family, member L antibody Histone gene cluster 1, H4 antibody Histone gene cluster 1, H4D antibody Histone gene cluster 1, H4E antibody Histone gene cluster 1, H4K antibody Histone gene cluster 4, H4 antibody Histone gene cluster 4, H4 histone antibody Histone H4 antibody histone IV, family 2 antibody
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Acetylated