Product Description

Histone H4 (MonoMethyl-R17) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67566-50, BS67566-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Modμlation of chromatin structure plays an important role in the regμlation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin . The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination . These modifications occur in response to various stimμli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression . In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 . Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms . Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase .

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: H4/A, H4FA, H4/I, H4FI, H4/G, H4FG, H4/B, H4FB, H4/J, H4FJ, H4/C, H4FC, H4/H, H4FH, H4/M, H4FM, H4/E, H4FE, H4/D, H4FD, H4/K, H4FK, H4/N, H4F2, H4FN, HIST2H4, H4/O, H4FO, Histone H4

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a Mono-methylation site at R17. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Histone H4 with a Mono-methylation site at R17 protein.

Molecular Weight: ~ 15 kDa

Pathway: Histone Lysine Methylation Pathway

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P62805

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only