HLA-A/ B/ C Polyclonal Antibody
Catalogue Numbers: BS60609-50, BS60609-100
Sizes: 50µl, 100µl
Alternative Name: HLA class I histocompatibility antigen, A-68 alpha chain; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68; HLA-A; HLAA
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01891/P01892/P04439/P05534/ P10314/ P10316/ P13746/ P16189/ P18462/ P30443/ P30450/ P30453/ P30455/ P30456/ P30457/ P30512/ Q09160
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A, -B and -C encode membrane anchored heavy chains which heterodimerize with a light chain (b-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-A, -B and -C alleles.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: HLA-A/ B/ C polyclonal antibody detects endogenous levels of HLA-A/ B/ C protein.
Bioworld Molecular Weight: ~ 41 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of HLA-A/ B/ C polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human HLA-A/ B/ C
Conjugate: Unconjugated
Modification: Unmodified