HLA Class 1 ABC Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9271-50, MB9271-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01889 Human;P30443 Human;P30499 Human
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: WB:1:500-1:2,000
ICC:1:50
IHC:1:50-1:200
Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: HLA Class 1 ABC monoclonal antibody detects endogenous levels of HLA Class 1 ABC protein.
Bioworld Molecular Weight: 40 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HLA Class 1 ABC on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature., ICC staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HLA Class 1 ABC at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Alternative Name: HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC, Antigen presenting molecule, HLA class I histocompatibility antigen, A 1 alpha chain, HLAA, Leukocyte antigen class I A, Major histocompatibility complex, class I, A, MHC class I antigen HLA A heavy chain, AS, HLA B protein, HLA class I histocompatibility antigen, B alpha chain, HLA-B, HLA-B histocompatibility type, HLAB, leukocyte antigen class I-B, lymphocyte antigen, major histocompatibility complex, class I, B, MHC class I antigen GN00104, MHC class I antigen HLA-B heavy chain, MHC class I antigen SHCHA, MHC Class I HLA heavy chain, MHC HLA-B cell surface glycoprotein, MHC HLA-B transmembrane glycoprotein, SPDA1, 1C07, Cw-7 alpha chain, D6S204, HLA class I histocompatibility antigen, HLA class I histocompatibility antigen, C alpha chain, HLA class I histocompatibility antigen, Cw-1 alpha chain, HLA JY3, HLA-C, HLA-C histocompatibility type, HLA-Cw, HLA-JY3, HLAC, HLC C, HLC-C, human leukocyte antigen-C alpha chain, major histocompatibility antigen HLA-C, major histocompatibility complex, class I, C, MHC class I antigen, MHC class I antigen Cw 1, MHC class I antigen Cw*7, MHC class I antigen heavy chain HLA-C, PSORS1 antibody
Immunogen: Recombinant protein within Human HLA Class 1 ABC aa 120-310.
Conjugate: Unconjugated
Modification: Unmodified