Product Description

HLA-DP Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65851-50, MB65851-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecμles are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecμles, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecμles and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecμle that presents an antigen, three MHC class II molecμles (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecμles until primary high affinity antigenic peptides are bound. The MHC II molecμle bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecμles is regμlated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regμlation of antigen loading into MHC II molecμles, increased acidification produces increased proteolysis and efficient peptide loading.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IF, FC

Alternate Names: HLA-DP1A, HLASB, HLA class II histocompatibility antigen DP alpha 1 chain, DP(W3), DP(W4), HLA-SB alpha chain, MHC class II DP3-alpha, MHC class II DPA1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Human NALM6 Leukemia Cells

Dilution: IF (1/50 - 1/200)

Purification: The monoclonal antibody was affinity-purified from Mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes human HLA-DP

Molecular Weight: ~ 29 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P20036

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Research Use Only