Product Description

HLA-DQA1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-60655-60, E-AB-60655-120, E-AB-60655-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: HLA-DQA1

Target Synonym: HLA-DQA1; CELIAC1; DQ-A1; HLA-DQA; CD; GSE; class II

Research Areas: Cancer, Immunology

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P01909

Background: HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human HLA-DQA1 (NP_002113.2).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200

Calculated MW: 27kDa

Observed MW: 28kDa-37KD

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only