Product Description

HLA-DRB4 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-90065-60, E-AB-90065-120, E-AB-90065-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: HLA-DRB4

Target Synonym: HLA-DRB4; DR4; DRB4; HLA-DR4B; major histocompatibility complex; class II; DR beta 4

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P13762

Background: HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human HLA-DRB4

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:1000-1:4000

Calculated MW: 29kDa

Observed MW: 29kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only