Product Description

HM74 (T327) Polyclonal Antibody

Catalogue Numbers: BS2605-50, BS2605-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49019/Q8TDS4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily. In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that produce proteins, namely HM74A and HM74 (or HM74B), which are 96% homologous. In mice and rats, only one gene encodes the HM74 protein (Gpr109a). HM74 is a G(i) protein-coupled receptor that mediates the metabolic effects of nicotinic acid. Localizing to the cell membrane, HM74 is highly expressed in adipocytes, immune cells and spleen. Like all members of the GPCR superfamily, HM74 contains seven transmembrane domains. HM74 lacks the N-linked glycosylation sites near the N-terminus that are present in other GPCR family members.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HM74 (T327) polyclonal antibody detects endogenous levels of HM74B protein. This antibody also recognizes HM74A protein.

Extra Notes: Western blot (WB) analysis of GPR109B pAb at 1:500 dilution
Lane1:PMVEC whole cell lysate(40ug)
Lane2:SP2/0 whole cell lysate(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:Myla2059 whole cell lysate(40ug)
Lane5:MCF-7 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Hydroxycarboxylic acid receptor 3; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; Niacin receptor 2; Nicotinic acid receptor 2; HCAR3; GPR109B; HCA3; HM74B; NIACR2; Hydroxycarboxylic acid receptor 2; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptor; HCAR2; GPR109A; HCA2; HM74A; NIACR1

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human HM74.

Conjugate: Unconjugated

Modification: Unmodified