Product Description

HMG14(Phospho-Ser21)Polyclonal Antibody

Catalogue Numbers:BS65220-50, BS65220-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P05114

Host: Rabbit

Reactivity: Human

Applications: IHC-p, IF/ICC, ELISA

All Applications: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Background: high mobility group nucleosome binding domain 1(HMGN1) Homo sapiens The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Phospho-HMG-14 (S21) Polyclonal Antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide., Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HMG14 (Phospho-Ser21) Antibody, Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.

Alternative Name: HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1

Immunogen: The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59

Conjugate: Unconjugated

Modification: Phosphorylation