Product Description

HMGB1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10355-50, MB10355-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P09429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HMGB1 in K562, rat Brain, C6, 3T3, Hela lysates using HMGB1 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using HMGB1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Immunogen: A synthetic peptide of human HMGB1

Conjugate: Unconjugated

Modification: Unmodified