Product Description

HMGB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90650-50, BS90650-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:50-1:100

Background: Like the histones, HMGB1, also known as high-mobility group protein 1 (HMG-1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 is secreted by immune cells through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. In recent research, HMGB1 has been reported as a novel biomarker for human ovarian cancer.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HMGB1 polyclonal antibody detects endogenous levels of HMGB1 protein.

Bioworld Molecular Weight: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: F9
Lane 4: A549
Lane 5: PC12, ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HMG-1, Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1, HMGB1, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified