HMGB1 Rabbit Monoclonal Antibody-BS9816M

HMGB1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9816M-50, BS9816M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:5000
IHC/ICC/IF: 1:50-1:200
FC: 1:10-1:100

Background: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Research suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, ATF-2, c-Jun and members of the NFκB family to activate transcription.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HMGB1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HMGB1 Rabbit mAb at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: PC12 whole cell lysate
Lane 3: F9 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB1 antibody. Counter stained with hematoxylin., ICC staining HMGB1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue).

Alternative Name: High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified