Product Description

HMGB4 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-14128-60, E-AB-14128-120, E-AB-14128-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: HMGB4

Target Synonym: High mobility group box 4; High mobility group protein B4; HMG2 like

Research Areas: Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q8WW32

Background: High mobility group protein B4is atranscription factorthat in humans is encoded by theHMGB4gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.

Concentration: 0.2 mg/mL

Immunogen: Recombinant protein of human HMGB4

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification Method: Affinity purification

Dilution: WB 1:200-1:1000

Calculated MW: 22kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only