HNF4α Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90653-50, BS90653-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P41235(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:100
Background: HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HNF4α polyclonal antibody detects endogenous levels of HNF4α protein.
Bioworld Molecular Weight: 53 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HNF-4-alpha on human kidney lysates using anti-HNF-4-alpha antibody at 1/1,000 dilution., ICC staining HNF-4-alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HNF-4-alpha, TCF-14, HNF4A, FLJ39654, FRTS4, Hepatic nuclear factor 4 alpha, Hepatocyte nuclear factor 4 alpha, Hepatocyte nuclear factor 4, Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF 4, HNF-4-alpha, HNF4, HNF4A, HNF4A, HNF4a7, HNF4a8, HNF4a9, Hnf4alpha, HNF4alpha10/11/12, MODY 1, MODY, MODY1, NR2A1, NR2A21, Nuclear receptor subfamily 2 group A member 1, OTTHUMP00000031060, OTTHUMP00000031062, TCF 14, TCF, TCF-14, TCF14, Tcf4, Transcription factor 14, hepatic nuclear factor, Transcription factor 14, Transcription factor HNF 4, Transcription factor HNF-4, Transcription factor HNF4,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified