hnRNP K Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90656-50, BS90656-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P61978(Human) P61979(Mouse) P61980(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: hnRNP K polyclonal antibody detects endogenous levels of hnRNP K protein.
Bioworld Molecular Weight: 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of hnRNP K on different lysates using anti-hnRNP K antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: NIH/3T3, ICC staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: hnRNP K, TUNP, HNRNPK, CSBP, dC stretch binding protein, FLJ41122, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, HNRNPK, HNRPK, HNRPK, Transformation up regulated nuclear protein, Transformation up-regulated nuclear protein, Transformation upregulated nuclear protein, TUNP,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified