Product Description

hnRNP L (G58) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1931-50, BS1931-100

Sizes: 50µl, 100µl

Swiss-Prot: P14866

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing and mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II). There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the majorconstituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB) and its homolog hnRNP L bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV).

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: hnRNP L (G58) polyclonal antibody detects endogenous levels of hnRNP L protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of hnRNP L (G58) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (10ug)
Lane 2: A549 whole cell lysate (10ug)
Lane 3: K562 whole cell lysate (10ug)
Lane 4: The Uterus tissue lysate of Rat (20ug)
Lane 5: The Brain tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of hnRNP L (G58) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Heterogeneous nuclear ribonucleoprotein L; hnRNP L; HNRNPL; HNRPL; P/OKcl.14

Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human hnRNP L.

Conjugate: Unconjugated

Modification: Unmodified