Product Description

hnRNP U Monoclonal Antibody

Catalogue Numbers: MB66525-50, MB66525-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q00839

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: hnRNP U, also known as scaffold attachment factor A (SAF-A) or SP120, was first described as a nuclear matrix protein that contributes to RNA metabolism. hnRNP U has been shown to bind pre-mRNAs and contributes to splicing by controlling U2 snRNP maturation . Furthermore, hnRNP U has been shown to bind chromatin to help regulate its structure during interphase. During prophase, hnRNP U becomes phosphorylated by Aurora-B, which evicts hnRNP U and the chromatin-associated RNAs bound to it, a process critical to proper chromosome alignment. hnRNP U plays a role in the chromatin localization of Xist RNA, and loss of hnRNP U in ES cells fail to achieve an inactivated X chromosome. hnRNP U has also been shown to play a key role in immunity, functioning as a viral dsRNA sensor. Upon viral recognition, hnRNP U oligomerizes and activates numerous antiviral genes, including IFNB1 . Loss of function variants of hnRNP U have been shown to cause early onset epilepsy and intellectual disorders.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of hnRNP U protein.

Extra Notes: Western blot analysis of hnRNP U expression in K562 (A), Jurkat (B), Hela (C) whole cell lysates.

Bioworld Molecular Weight: ~ 125 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: HNRPU; SAFA; U21.1; Heterogeneous nuclear ribonucleoprotein U; hnRNP U; Scaffold attachment factor A; SAF-A; p120; pp120

Immunogen: Purified recombinant human hnRNP U protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified