hnRNP U Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90658-50, BS90658-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q00839(Human) Q8VEK3(Mouse) Q6IMY8(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IF, IHC
All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: hnRNP U polyclonal antibody detects endogenous levels of hnRNP U protein.
Bioworld Molecular Weight: 120 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HnRNP U on different cell lysates using anti-HnRNP U at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: SH-SY-5Y
Lane 3: K562, ICC staining HnRNP U in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: hnRNP U, SAF-A, HNRNPU, C1orf199, HNRPU, SAFA, U21.1, Heterogeneous nuclear ribonucleoprotein U, hnRNP U, hnRNP U protein, HNRNPU, hnRNPU protein, HNRPU, HNRPU, p120, p120 nuclear protein, pp120, SAF A, SAF-A, SAFA, Scaffold attachment factor A, U21.1,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified