hnRPD(Phospho-Ser83)Polyclonal Antibody-BS65398

hnRPD(Phospho-Ser83)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65398-50, BS65398-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q14103

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF/ICC, ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Background: heterogeneous nuclear ribonucleoprotein D(HNRNPD) Homo sapiens This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-hnRNP D0 (S83) Polyclonal Antibody detects endogenous levels of hnRNP D0 protein only when phosphorylated at S83.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using hnRPD (Phospho-Ser83) Antibody, Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis of lysates from HUVEC and 293 cells, using hnRPD (Phospho-Ser83) Antibody. The Lane on the right is blocked with the phospho peptide.

Alternative Name: HNRNPD; AUF1; HNRPD; Heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; AU-rich element RNA-binding protein 1

Immunogen: The antiserum was produced against synthesized peptide derived from human hnRPD around the phosphorylation site of Ser83. AA range:49-98

Conjugate: Unconjugated

Modification: Phosphorylation