HO-1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90659-50, BS90659-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09601(Human) P14901(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC IHC
All Applications: WB:1:500-1:1,000
ICC:1:500
IHC:1:50-1:200
Background: Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
Purification and Purity: Peptide affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: HO-1 polyclonal antibody detects endogenous levels of HO-1 protein.
Bioworld Molecular Weight: 33 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution., ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HO-1, HMOX1, HO, HO1, 32 kD, bK286B10, D8Wsu38e, heat shock protein 32 kD, heat shock protein 32kD, Heat shock protein, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, HMOX 1, Hmox, Hmox1, HMOX1, HO 1, HO, HO-1, HO1, Hsp32,
Immunogen: Synthetic peptide of N-terminal human Heme Oxygenase 1 (HO-1).
Conjugate: Unconjugated
Modification: Unmodified