HP1γ Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90661-50, BS90661-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13185(Human) P23198(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC FC IP,
All Applications: WB:1:500
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HP1γ polyclonal antibody detects endogenous levels of HP1γ protein.
Bioworld Molecular Weight: 20 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
Alternative Name: HP1 gamma, CBX3, CBX 3, CBX3, CBX3, Chromobox homolog 3 (HP1 gamma homolog, Drosophila), Chromobox homolog 3, Chromobox protein homolog 3, GAMMA, HECH, Heterochromatin like protein 1, Heterochromatin protein 1 homolog gamma, Heterochromatin protein HP1 gamma, HP1 gamma, HP1 gamma homolog, HP1Hs gamma, Modifier 2 protein,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified