HRP-conjugated Donkey Anti-Mouse IgG (H+L)
Size: 100μg
Catalogue Number: AS033-100
Citations, Manuals and MSDS Available upon request.
Background: Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence) . They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Category: Secondary Antibodies
Applications: WB, IHC-P
Cross-reactivity: Mouse
Observed Molecular Weight: 25kDa (Light chain), 55kDa (Heavy chain) /36kDa
Species: Mouse
Source: Donkey
Isotype: Horseradish peroxidase conjugated IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:10000 - 1:20000 IHC-P, 1:500 - 1:1000
NCBI Alias: Donkey Anti-Mouse
Research Use Only