Product Description

Hrs (R481) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2448-50, BS2448-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14964

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a zinc-finger protein that interacts with STAM and undergoes tyrosine phosphorylation in response to IL2, CSF2 or HGF. Hrs is involved in intracellular trafficking and signal transduction and is associated with early endosomes. H contains a phosphatidylinositol 3-phosphate-binding domain that contributes to its endosomal targeting, where Hrs colocalizes with Clathrin via a Clathrin box motif at the carboxy terminus of Hrs. Hrs is essential for ventral folding morphogenesis and shares structual similarity to the yeast protein Vps27p, which is involved in vacuolar protein sorting. The human Hrs gene, which maps to chromosome 17q25, enodes a 777 amino acid protein. In Schwann cells, Hrs colocalizes at endosomes with the tumor suppressor protein schwannomin, suggesting a role for schwannomin in Hrs-mediated cell signaling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Hrs (R481) polyclonal antibody detects endogenous levels of Hrs protein.

Bioworld Molecular Weight: ~ 86 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Hrs (R481) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of Hrs (R481) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Hrs (R481) pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Hrs (R481) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110; HGS; HRS

Immunogen: Synthetic peptide, corresponding to amino acids 461-510 of Human Hrs.

Conjugate: Unconjugated

Modification: Unmodified