HSC 70 Rabbit Monoclonal Antibody-BS9859M

HSC 70 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9859M-50, BS9859M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11142

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 (heat shock cognate protein 70) play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HSC 70 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSC 70 Rabbit mAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: PC12 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsc70 antibody.Counter stained with hematoxylin., ICC staining Hsc70 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LAP1; LPS-associated protein 1; HSPA8; HSC70; HSP73; HSPA10

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified