Product Description

HSD11B1 Polyclonal Antibody

Catalogue Numbers: BS5588-50, BS5588-100

Sizes: 50µl, 100µl

Alternative Name: Corticosteroid 11-beta-dehydrogenase isozyme 1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1; HSD11B1; HSD11; HSD11L; 11β-HSD1; 11β HSD1; 11βHSD1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28845

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Glucocorticoid hormone action in target tissues is modulated by 11b-hydroxysteroid dehydrogenase (11b-HSD), which catalyzes the interconversion of hormonally active C11-hydroxylated corticosteroids (cortisol, corticosterone) and their inactive C11-keto metabolites (cortisone, 11-dehydrocorticosterone). At least two isoforms of 11b-HSD exist: a low-affinity NADP-dependent dehydrogenase/oxoreductase (11b-HSD1) and a high-affinity NAD-dependent dehydrogenase (11b-HSD2). The glycosylated 11b-HSD1 protein activates cortisol from cortisone, which is widely expressed in mammals, and is most highly expressed in the liver. 11b-HSD1 may play a role in glucose homeostasis and pathogenesis of a number of disorders including insulin resistance and obesity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSD11B1 polyclonal antibody detects endogenous levels of HSD11B1 protein.

Bioworld Molecular Weight: ~ 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSD11B1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: H9C2 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 43-88 of Human HSD11B1.

Conjugate: Unconjugated

Modification: Unmodified