Product Description

HSD3B7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65304-50, BS65304-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q9H2F3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA

All Applications: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Background: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7(HSD3B7) Homo sapiens This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: 3β-HSD7 Polyclonal Antibody detects endogenous levels of 3β-HSD7 protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using 3β-HSD7 Polyclonal Antibody diluted at 1：1000, Western Blot analysis of COLO205 cells using 3β-HSD7 Polyclonal Antibody diluted at 1：1000, Western blot analysis of lysates from HeLa, MCF-7, and COLO cells, using HSD3B7 Antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from Jurkat cells using HSD3B7 antibody.

Alternative Name: HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; C(27) 3-beta-HSD; Cholest-5-ene-3-beta; 7-alpha-diol 3-beta-dehydrogenase

Immunogen: The antiserum was produced against synthesized peptide derived from human HSD3B7. AA range:121-170

Conjugate: Unconjugated

Modification: Unmodified