Product Description

HSF1 (phospho-S303) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4795-50, BS4795-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regμlation. HSF1 and HSF2 are upregμlated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF

Alternate Names: Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSF1, HSTF 1, HSTF1,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Serine 303.

Dilution: IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-HSF1 (S303) polyclonal antibody detects endogenous levels of HSF1 protein only when phosphorylated at Ser303.

Molecular Weight: ~ 82 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q00613

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only