HSF1 (Phospho-S326) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94019-50, BS94019-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q00613(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC, IHC, IP, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HSF1 (Phospho-S326) polyclonal antibody detects endogenous levels of HSF1 protein only when phosphorylated at S326.
Bioworld Molecular Weight: 82 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of p-HSF1(S326) on different lysates using anti-p-HSF1(S326) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: BT20
Lane 3: AGS, ICC staining p-HSF1(S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HSF 1, HSTF 1, HSF1, Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSF1, HSTF 1, HSTF1,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser326 of human HSF1.
Conjugate: Unconjugated
Modification: Phosphorylation