Product Description

HSF2BP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS72638-50, BS72638-100

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: HSF2 binding protein (HSF2BP) associates with HSF2. The interaction occurs between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Extra Notes: Western blot analysis of extracts of mouse testis, using HSF2BP antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Immunofluorescence analysis of L929 cells using HSF2BP Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Bioworld Molecular Weight: 38kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: HSF2BP

Immunogen: Recombinant fusion protein of mouse HSF2BP(NP_083178.1).

Conjugate: Unconjugated

Modification: Unmodified