Product Description

HSL (Phospho-S853) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94037-50, BS94037-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05469(Human) Q6NSL7(Human) P54310(Mouse) P15304(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:1,000-1:5,000

Background: HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of phospho -Hormone sensitive lipase (S853) on human skeletal muscleLysates using anti- phospho -Hormone sensitive lipase (S853) antibody at 1/1,000 dilution.

Bioworld Molecular Weight: 84 kDa

Specificity: HSL (Phospho-S853) polyclonal antibody detects endogenous levels of HSL protein only when phosphorylated at S853.

Alternative Name: HSL, Lipe, Hormone sensitive lipase, Hormone sensitive lipase testicular isoform, Hormone-sensitive lipase, HSL, LHS, Lipase hormone sensitive, LIPE, LIPS,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser853 of human Hormone sensitive lipase.

Conjugate: Unconjugated

Modification: Phosphorylation