Product Description

HSP27 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0141-50, MB0141-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04792

Host: Mouse

Reactivity: Human, Monkey

Applications: WB, ICC

All Applications: WB: 1:1000
ICC: 1:100~500

Background: Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HSP27 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 23KDa
Observed band size: 27KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of HSPB1 in Hela, HsHela, A431, COS7, LNCAP and MCF7 cell lysates and using HSPB1 antibody (1:1000 diluted)., Immunocytochemistry stain of Hela using HSPB1 antibody (1:300).

Alternative Name: Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28

Immunogen: Recombinant full length Human HSP27.

Conjugate: Unconjugated

Modification: Unmodified