Product Description

HSP27 (phospho-S82) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0244-50, AP0244-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Hsp27, also referred to as the Estrogen-Regμlated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecμlar weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a usefμl marker in classifying various hormone sensitive tumors.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regμlated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 82.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HSP27 (phospho-S82) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser82.

Molecular Weight: ~ 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04792

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only