HSP27 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90667-50, BS90667-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04792(Human) P14602(Mouse) P42930(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HSP27 polyclonal antibody detects endogenous levels of HSP27 protein.
Bioworld Molecular Weight: 27 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Hsp27 on A549 cells lysates using anti-LRP1 antibody at 1/1,000 dilution., ICC staining Hsp27 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HspB1, HSP 27, SRP27, HSPB1, Heat shock 27kDa protein, 28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, HSP27, Hsp28, HspB1, HSPB1, SRP27, Stress responsive protein 27, Stress-responsive protein 27,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified